Reliability of a Compact and Portable Chemiluminescence Detector

Abstract

Two reliability issues (the lowering of voltage and the recovery of Teflon) for a compact chemiluminescence detector for glucose measurement based on a single planar transparent EWOD (electrowetting-on-dielectrics) device are studied. Several dielectrics for lowering the manipulation voltage are investigated and a low voltage of 20-27V is realized. An on-chip heater is designed and manufactured for restore the damage to the hydrophobic surface of the EWOD following the chemiluminescence reaction. Glucose measurements show detector sensitivity reaches 0.12V/M, with a detection range of 1M to 20mM and a detection limit of 1M, indicating it’s the detector’s potential as a portable immuno-detector offering prompt response and low cost measurement compared with expensive and bulky traditional instruments.